Research
2022
Profile diversity of galacto-oligosaccharides from disaccharides to hexasaccharides by porous graphitic carbon liquid chromatography-orbitrap tandem mass spectrometry
https://www.sciencedirect.com/science/article/abs/pii/S030881462201113X?via%3Dihub
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An improved PGC-LC-Orbitap MS/MS method revealed the diversity of GOS with DP2-DP6.
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The system separated and identified 58 linear and 10 branched GOS.
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Fifteen major group components with DP2-DP5 comprised over 65% of total GOS content.
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Non-reducing GOS components with DP2-DP6 comprised 2.8–7.6% of total GOS content.
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The major structural diversity occurred in DP3-DP5 GOS.
2020
Preparation and FTIR, Raman and SEM characterizations of konjac glucomannan-KCl electrogels
https://www.sciencedirect.com/science/article/pii/S0308814620311511?dgcid=rss_sd_all
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KCl interacts with konjac glucomannan to form the structure
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Konjac glucomannan chains break and partially deacetylate under alternating current.
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Alternating current induces the formation of konjac glucomannan-KCl electrogel.
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Konjac glucomannan-KCl electrogel possesses an ununiform porosity structure.
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Viscoelastic moduli of the gels depend on KCl and konjac glucomannan concentration, voltage and electric processing time.
2020
Biotransformation of mogrosides from Siraitia grosvenorii by Ganoderma lucidum mycelium and the purification of mogroside III E by macroporous resins
https://www.sciencedirect.com/science/article/pii/S1021949819300493#undfig1
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The first paper providing processes for the preparation of Mogroside III E from a biotransformed mogroside mixtures.
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Mogroside III E is identified as the major metabolite of the Ganoderma lucidum mycelium during mogrosides biotransformation.
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The one step and one column separation technique provide a five-fold increase in the purification factor from 11.7% to 55.1%.
2018
Application of thermal stability difference to remove flammutoxin in fungal immunomodulatory protein, FIP-fve, extract from Flammulina velutipes
https://www.sciencedirect.com/science/article/pii/S1021949818300267
Fungal immunomodulatory protein (FIP-fve) is a potential functional food ingredient. However, undesirable component flammutoxin (FTX) would occur in the extracted fraction of FIP-fve. In this paper, an application of heating processing instead of the intensive separation process was employed in fractionation of FIP-fve, meanwhile, exclusion of FTX was reached.
2018
Characteristics of fucose-containing polysaccharides from submerged fermentation of Agaricus blazei Murill
https://www.sciencedirect.com/science/article/pii/S1021949817301436
Fucose is one of important residues of recognition pattern for many immune cells. In this study, we characterized bioactive fucose-containing acidic polysaccharides from submerged fermentation of Agaricus blazei Murill. Regarding bioactivity, removal of the terminal l-fucosyl residues reduced the TNF-α cytokine stimulating activity of the polysaccharides in a RAW 264.7 macrophage cell-line test, whereas NF-κB and TLR4 affected the polysaccharide-induced TNF-α production.
2014
Concentration Variation and Molecular Characteristics of Soluble (1,3;1,6)-β-D-Glucans in Submerged Cultivation Products of Ganoderma lucidum Mycelium
https://pubs.acs.org/doi/10.1021/jf404533b
(1,3)-β-D-Glucans with (1,6)-β-D-glucosyl branches are bioactive polysaccharides in fruiting bodies and mycelia of Ganoderma lucidum, a mushroom used in traditional Chinese medicine. Submerged cultivation of mycelium is one of the more efficient means of generating polysaccharides from this fungus. Twelve mycelium samples examined in this study demonstrated the quantitative and qualitative molecular characteristics of soluble (1,3;1,6)-β-D-glucans. Using the high aggregating tendency of these molecules, (1,3;1,6)-β-D-glucans were successfully purified via fractional precipitation with 35% (v/v) ethanol. (1,3;1,6)-β-D-Glucan was proposed as a putative bioactive marker for immunomodulation because it was the most abundant polysaccharide in G. lucidum mycelium products to stimulate macrophage RAW 264.7 cells to release TNF-α.
Anoectochilus formosanus (Orchidaceae) is a folk medicine in Asia. This study investigated the in vivo and in vitro prebiotic effects of an aqueous extract of A. formosanus (SAEAF) and of an indigestible polysaccharide (AFP) isolated from SAEAF. Chemical analyses showed AFP was mainly composed of arabinogalactan type II (AG-II).Through a bioactivity-guided separation strategy, AFP, a polysaccharide from A. formosanus, was demonstrated to be a prebiotic that has a positive health effect on gut microbiota. Furthermore, a type II arabinogalactan from AFP could reduce bone loss through its prebiotic effect in vivo and in vitro. A broadspectrum antibiotic, streptomycin, was used for clearing the relationship of prebiotic and anti-osteoporosis effects. This study investigated a new prebiotic which could enhance bone health efficiently and be used in small dosage.
2012
Characterization and prebiotic activity of aqueous extract and indigestible polysaccharide from Anoectochilus formosanus
https://pubs.acs.org/doi/10.1021/jf3018832
2013
The prebiotic arabinogalactan of Anoectochilus formosanus prevents ovariectomy-induced osteoporosis in mice
https://www.sciencedirect.com/science/article/pii/S1756464613001540
2013
Biotransformation of Mogrosides from
Siraitia grosvenorii Swingle by Saccharomyces cerevisiae
https://pubs.acs.org/doi/10.1021/jf402058p
Mogrosides are a group of triterpenoidal saponins from the fruit of Siraitia grosvenorii Swingle; they are intensely sweet and have consequently been used as a substitute for sugar by the food industry. The lack of efficient methods to produce specific mogrosides has hindered investigation of the relationship between their structure and bioactivity. Here, we attempt to selectively convert the major saponin mogroside V, a mogrol pentaglucoside, into mogroside III E, a triglucoside, via the β-glucosidases of the budding yeast Saccharomyces cerevisiae. This paper demonstrates that yeast knockout mutants are a valuable tool for saponin modification and for studying the specificity of glucosidase function.
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